A tissue fastener production method of clamping two living tissues together by bringing the two living tissues into close contact includes the following steps: fixing one end of a wire material made of a metal to a shaft body, and winding the wire material around an outer periphery of the shaft body while twisting the wire material round an axis of the wire material, thereby forming a coil on which a predetermined force is exerted in the winding direction; heat treating the wire material to impart superelasticity thereto; and deforming the wire material to which the superelasticity is imparted in a range in which the wire material is elastically deformable, and reversing the winding direction of the coil in a direction reverse to the winding direction of the coil wound in the step of forming the coil so as to obtain an initial tension corresponding to the predetermined force.
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